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), which is identical angular position as being the covalent connections in between the ChVLig OB area as well as strands of your latch. So, Mother nature has located extremely distinct structural solutions to the problem of DNA envelopment by ATP-dependent ligases.
). The protein-DNA interface involves considerable DNA contacts because of the NTase, OB, and HhH domains more than a 19-bp section of duplex DNA centered with regard to the nick (supplemental Fig. S3). The NTase domain binds to your damaged DNA strands at and flanking the nick; the OB domain contacts the continuous template strand surrounding the nick; as well as HhH domain binds the two strands over the minimal groove at the periphery from the footprint.
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). In the free ChVLig-AMP, the OB area is reflected far from the NTase domain to totally expose the DNA-binding floor earlier mentioned the AMP-binding pocket. The peptide section that is certainly destined to become the latch is disordered during the cost-free ligase and sensitive to proteolysis, but this section is protected from proteolysis when ChVLig binds to nicked DNA.
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De igual manera, dejaron claro que para la entrada al estadio se utilizará la aplicación Quentro en donde se podrán recibir y transferir las entradas.
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